Composite

Part:BBa_K4340605:Design

Designed by: Chein Yueh Liu   Group: iGEM22_PuiChing_Macau   (2022-09-29)


sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1283
    Illegal PstI site found at 5809
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1238
    Illegal PstI site found at 5809
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1349
    Illegal BamHI site found at 1205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1283
    Illegal PstI site found at 5809
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1283
    Illegal PstI site found at 5809
    Illegal NgoMIV site found at 1381
    Illegal NgoMIV site found at 2969
    Illegal NgoMIV site found at 3129
    Illegal NgoMIV site found at 3483
    Illegal AgeI site found at 752
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5635
    Illegal SapI site found at 4552


Design Notes

/


Source

sfGFP(BBa_K2762014) and pET11a vector

References