Composite
Part:BBa_K4340605:Design
Designed by: Chein Yueh Liu Group: iGEM22_PuiChing_Macau (2022-09-29)
sfGFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1283
Illegal PstI site found at 5809 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1238
Illegal PstI site found at 5809 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1349
Illegal BamHI site found at 1205 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1283
Illegal PstI site found at 5809 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1283
Illegal PstI site found at 5809
Illegal NgoMIV site found at 1381
Illegal NgoMIV site found at 2969
Illegal NgoMIV site found at 3129
Illegal NgoMIV site found at 3483
Illegal AgeI site found at 752 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5635
Illegal SapI site found at 4552
Design Notes
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Source
sfGFP(BBa_K2762014) and pET11a vector